Generuler 1 kb dna ladder thermo fisher scientific. Pdf dna ladder is commonly used to determine the size of dna fragments by. Twofold serial dilutions of 1 kb plus dna ladder invitrogen were loaded in the amounts of 200 ng, 100 ng. Pdf construction of a synthetic vector for preparation. Do not heat the trackit 1kb plus dna ladder before loading.
The product summary sheet, or data card, includes details for how to use. Fermentas, and nuclease free water new england biolabs, ipswich, ma, u. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder. The ladder is supplied with 6x dna loading dye, and it can be used in dna sizing and approximate quanitification applications. Thermo scientific ogeneruler 1 kb dna ladder, readyto. Thermo scientific fermentas fastdigest enzymes are an advanced line of restriction enzymes for rapid dna. Pdf straightforward procedure for laboratory production of dna. Thermo scientific generuler 1 kb dna ladder perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this mixture of chromatography. Generuler 1kb ladder fermentas, 4 100bp ladder new. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to.
Both 100 bp and 1 kb ladders can either be purchased from commercial sources or generated in. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. The ladder is a mixture of chromatographypurified individual dna fragments. Thermo scientific generuler 1 kb dna ladder, readytouse. From routine analysis to pioneering discoveries, our innovations help scientists solve. This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. Fermentas generuler 1 kb dna ladder readytouse, 5 x 50. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. Product information thermo scientific generuler 1 kb. Pdf creator software latest version free download 16rqrz pdf creator software latest version free download 3d39b66ab9.
Generuler 1 kb plus dna ladder thermo fisher scientific. Thermo scientific fermentas fastdigest restriction enzymes. Generuler 1 kb dna ladder, readytouse catalog number sm03, sm0314 pub. Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. The dna ladder consists of proprietary plasmids which.
Use the loading dye solutions supplied with every fermentas dna ladder. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. Generuler 1 kb plus dna ladder can be labeled radioactively with t4 polynucleotide kinase. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple. Fermentas generuler 1 kb dna ladder readytouse, 5 x 50 ug, price. Page 1 4 material safety data sheet prepared in accordance with eu directive 91155eec printed.
The blue dye migrates with 35 kb dna fragments in a 1 % agarose gel and has an excitation peak of 424 nm. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. Generuler 100 bp plus dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. The ladder is composed of fifteen chromatographypurified individual dna. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye.
Hyperladder 1kb with 1 % wv agarose gel, hyperladder 50bp with 1. L 01 restriction digestion with block cooler as incubation. Do not exceed the amount of electrophoresis tracking dyes used for sample ladder preparation. Straightforward procedure for laboratory production of dna ladder. This product is covered by one or more patents, trademarks andor s owned or controlled by new england biolabs, inc neb. Generuler 1 kb plus dna ladder thermo fisher scientific assets. Lanes 1 and 2 represent 100 bp and 1 kb dna ladders, respectively fermentas. June 2018 54 may 2018 87 april 2018 37 march 2018 32 february 2018 27 january 2018 25 december 2017 16 november 2017 4 please reload. Pdf straightforward procedure for laboratory production. Generuler 1 kb plus dna ladder 1250 25 x 50 g for 2500 applications, 0. Thermo scientific products help scientists around the world meet the challenges they face every day. Download a pdf containing pricing for our full product list. A molecularweight size marker, also referred to as a protein ladder, dna ladder, or rna.
Description thermo scientific generuler 1 kb dna ladder is. Generuler 1kb ladder fermentas, 4 100bp ladder new england biolabs. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye.
The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The ladder is composed of fourteen chromatographypurified. The epub format uses ebook readers, which have several ease of reading features already built in. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information.
This product was previously named the 2log dna ladder. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Generuler 1 kb plus dna ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Use the 1 kb plus dna ladder is to size linear doublestranded dna fragments from 100 bp to 12 kb. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. Please have a look into the related application note for more information about ladder. The ladder is composed of fifteen chromatographypurified.
Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Gel conditions are 1% agarose, 3 voltcm, and ethidium bromide stain. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. A molecularweight size marker in the form of a 1kb dna ladder in the rightmost lane, used in gel electrophoresis. Thermo scientific generuler 1 kb dna ladder, readytouse 2500bp, 0. A was added to eppendorf tubes to a final volume of 20. Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna ranging from 10 bp to 48. Construction of a synthetic vector for preparation of a 100 base pair dna ladder. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder. Product information thermo scientific generuler 1 kb dna. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The ppsu plasmids for generating dna molecular weight markers. Geldocit imaging system using gelred and gelgreen biotium.
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