Located in san diego, ca, we serve the international community of researchers with the highestquality used lab equipment at the lowest possible cost, for scientists by scientists. The detected fluorescence was displayed as arbitrary fluorescence units afu. Currently we have over 300 windows 710 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Plates were read on a fluorescent plate reader applied biosystems, cytofluor series 4000, foster city, ca. One hundred microliters of each reaction was transferred to a fluoronunc microtiter plate, and the fluorescent emission was measured in a cytofluor series 4000 fluorescence multiwell plate reader perseptive biosystems, framingham, ma or a dynex fluorite fluorescence multiwell plate reader dynex technologies, chantilly, va. Cells were induced to differentiate in the presence or absence of nu. Applied biosystems cytofluor series 4000 multiwell fluorescence reader users guide describes how to set up, use, and maintain the cytofluor series 4000 fluorescence multiwell plate reader. We supply almost any item you need in your lab balance, ovens, incubators, centrifuges, microscopes and many more. Applied biosystems november 2001 iii contents 1 about this manual 11 contents of the manual 11.
Please refer to the applied biosystems cytofluor manual for specific. Gmi provides new and remanufacturedtospecification gmi certified scientific instrumentation and instrument service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics, analytical laboratories, in the united states and in more than 50 countries. The plate was then placed in the cytofluor 4000 instrument, which had been set to 340 nm excitation490 nm emission and automatic mode, for 20 cycles or less, and the plate was read in a kinetic mode. Afus were determined as the average fluorescence of three consecutive measurements per well at a gain of approximately 50. Analytical evaluation of primer engineered multiplex. Please provide details about your project so that we can deliver a quote that is most appropriate to your needs. Applied biosystems assumes no responsibility for any errors that may appear in this document. Quantitative analysis of fluorescent caspase substrate. Nova biotech provides service and sales support for uvvis spectrophotometers, spectrofluorometers, microplate readers, ftir, pcr, hplc, fplc and temperature control instruments across the u.
Thermo applied biosystems cytofluor series 4000 multi. Testing abrasion, adhesion thickness, hardness or viscosity. Products generated using the abi prism snapshot multiplex kit can be. The entire system consists of a scanner and the cytofluor software for. Hla superfamily assignment is a predictor of immune response to cancer testis antigens and survival in ovarian cancer. The cytofluor 4000 multiwell plate reader can scan both tops and bottoms. Fluorescence excitation at 560 nm and emission at 590 nm was measured using a spectrofluorometer cytofluor 4000, perseptive applied biosystems, cambridge, ma, usa.
Viability was calculated as difference between the lysed and nonlysed replicates of treated divided by the same calculation in nontreated controls. Nitrite standards were prepared fresh and standard curves were constructed in the same media as samples. Perseptive biosystems inc annual report 10k item 1. These instruments have the necessary resolution and. New thermal cycler archives page 2 of 2 gmi trusted. University of pittsburgh financial disclosure report. Our offices and depot repair facilities are located in southern california. Nontransfected cells were used as a control to determine the background level.
The second manual in the set is the abi 3948 reference manual pn 4303111. Equipment for sale category or equipment item cytofluor iifluorescencemicroplatereader not found. The antimalarial artesunate also exerts profound anticancer activity. Electrostatic switches that mediate the phdependent. Perseptive biosystems cytofluor 4000 microplate reader. Abi 3948 nucleic acid synthesis and purification system. Samples and standards were read at 360 nm ex and 460 nm em with a gain setting of 90 on a cytofluor 4000 fluorescence plate reader, perseptive biosystems framingham, ma. Typically, the signaltonoise ratio obtained by using an endpoint analysis after the readings was at least threefold.
Perseptive applied biosystems cytofluor 4000 fluorescence and luminescence glow. Therefore, only the calcein fluorescence data were used. T164708 perseptive biosystems cytofluor 4000 multiwell plate reader mifsoc2tr. L of 3 n naoh, and immediately read by microplate reader perseptive biosystems, cytofluor series 4000, forster city, ca set for.
Laboratory equipment for sale scientific equipment repair. Without the manufacture and testing of the 4000 angstrom particle, and the recognition and publication of the positive. Cytofluor 4000 tc includes temperature cytofluor 4000 tr no temperature control fluorescence readers that come complete with pc software, line cord, manual, six days a week technical support and one year warranty. Versatile onboard software, upgraded electronics, and ability to add specialized. It is possible to search for the overlord device driver for the instrument of your choice. This fluorescence and bioluminescence reader quantifies soluble and cellassociated fluorescence. Its software runs on a pc operating under microsoft windows software. Abi prism and its design, applied biosystems, biolims, genescan, genotyper. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Macintosh 3948control software 112 applied biosystems synthesis support 112.
Check out the most popular applied biosystems products for genetic analysis. Novel activating mutation in the ret tyrosine kinase. Tests conducted in accordance with the guidelines of the environmental. Development of a cellbased highthroughput specificity. Microplate fluorescence plate readers mtx lab systems. Common tests include those used in newborn screening, determination of tsh, t4. The fluorescence is measured by a photomultiplier tube in a lightproof detection chamber.
Vcu department of biochemistry and molecular biology vcu medical center. Cathepsin d catd is a member of the pepsin family of aspartic peptidases. Its possible we can locate the instrument you are looking for. Lets go robotics offers a range of products and services to meet the varied needs of our customers. Synthesis and cytotoxicity of y 2 o 3 nanoparticles of. System includes a computer with cytofluor version 4. Biosystems offers a broad, extensive portfolio of clinical diagnostics products to streamline the work flow, enhance operational efficiency and improve patient treatment. Traditionally, catd was believed to play an important role in general proteolysis during bulk turnover at acidic ph. All instruments are reconditioned and include a 90 day warranty on parts and labor. The atpbinding cassette abc transporters abcb6 and abcb7 are also involved in iron homeostasis. Perseptive biosystems biochromatography software v3. Role of transferrin receptor and the abc transporters. The various product lines include a wide range of reliable instruments and reagents for clinical chemistry, coagulation, hematology, autoimmunity and serology. Characterization of nitric oxide production following.
Efficiency of eight different aav serotypes in transducing. To test the capacity of aav serotypes for efficient myocardial transduction, we used eight different vectors to infect cardiac muscle. Please fill in the form below to get a quote for the perseptive biosystems cytofluor 4000 microplate reader. Abi prism and the abi prism design, applied biosystems, genescan, genotyper. The unit also contains software to comply with the us food and drug administrations. Find amada, mazak, trumpf, terex, doosan, and genie for sale on machinio. In addition, we are the authorized sales and service providers for gilford and unico spectrophotometers. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Greater sensitivity and precision than other existing molecular tests.
This system enables you to scan data on soluble and cellassociated fluorescence. Testing equipment 7 accessory 1 calibration 1 calorimeter 1 colorimeter 0. Department of biochemistry and molecular biology at virginia commonwealth university. Vcu department of biochemistry and molecular biology. In order to get access to these contents, you must log in should you need a user id and password, please contact biosystems. Fluoroskan iperseptive biosystems cytofluor 4000bmg fluostar. The media was subsequently exchanged for pbs and the fluorescence from intact cells measured using a 96well fluorometer perceptive biosystems cytofluor equipped with the. Abi prism and its design, applied biosystems, genescan, genotyper, microamp, biolims, and biomerge are registered trademarks. Microsoft excel was used to perform t testing for statistical analysis of the data. When grinnell college framed its charter in the iowa territory of the united states in 1846, it set forth a mission to educate its students for the different professions and for the honorable discharge of. Fluorescence was detected with a cytofluor 4000 96well plate reader perceptive biosystems, framingham, mass. Applied biosystems 7500 fast software thank you thermo. Perceptive biosystems cytofluor 4000 perkin elmer life sciences winprep software. The company launched the cytofluor 4000 in 1996 with temperature control and enhanced features and is currently launching titerfluor fluorescence in vitro assays for use with the cytofluor 4000.
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