Isolation of potent biosurfactant producing bacteria from oil. Additionally, hydrophobic interaction chromatography and the replica plate technique are also rewarding methods. The identified bacteria bacillus subtilis and bacillus cereus which were isolated from water and soil respectively were found to possess biosurfactant producing. Screening and optimization of biosurfactant production by the. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran. Priyadharshini and latha, 2016 biosurfactant production from bacillus sp. Isolation of biosurfactant producing bacteria from oil. Characterization of biosurfactant produced by probiotic.
Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant producing bacteria. Isolation of biosurfactant producing bacteria from oil reservoirs. Screening and isolation of biosurfactant producing. The goal of this study was isolation and characterization of biosurfactant producing bacteria from caspian sea water. The aim of this study was to introduce palm kernel cake pkc as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation. Screening and isolation of biosurfactant producing bacteria. Pdf isolation of biosurfactant producing bacteria from petroleum.
Production of biosurfactant by bacillus licheniformis. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. This study was primarily based on the isolation and identification of biosurfactantproducing bacteria that could utilize palm kernel cake as a new major substrate. These 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. Possibility of occurrence of biosurfactant producers in such effluents collected from local tanneries was investigated in this study. Soil from automobile workshop and garages in the jaipur city, rajasthan, india. Enhanced biosurfactant production was carried out using olive oil as the carbon. The methodology was able to isolate biosurfactant producing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources. Isolation of biosurfactant producing exiguobacterium sp. The biosurfactant producing ability of the isolated strains was investigated by.
Pdf biosurfactants are microbial amphiphilic compounds which can reduce. Two microorganisms showed the highest biosurfactant production when grown on paraffin and glycerol as sole carbon. Total 30 bacterial strains were isolated from samples of soil contaminated with petroleum and its products. Research article isolation and identification of bacillus. Isolation of biosurfactant producing marine bacteria and characteristics of selected biosurfactant songklanakarin j. Ten out of thirty strains showed the biosurfactant activity. Isolation of biosurfactant producing bacteria soil samples were collected october 30th, 2018 from groundnut oil cake dumping sites at different areas of hyderabad, telangana, india. The biosurfactant producing ability of bacteria was investigated by oil displacement agar plate and emulsification index method. The bacterial colonies were then observed for the presence of clear zonearound the colonies. The objective of this study was to isolate and screening biosurfactant producing bacteria from petroleum oil contaminated terrestrial samples collected in bangkok, thailand.
These results are in agreement with the results that reported by other researcher. Apart from direct isolation of strains by diluting and plating, enrichment cultures with hydrophobic substrates are very promising for the isolation of biosurfactant producing microbes. Screening and isolation of biosurfactant producing bacteria from petroleum contaminated soil pradnya a. Original article characterization and phylogenetic analysis of biosurfactantproducing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand. Fortyfive of the isolates were positive for biosurfactant production, representing most of the soils tested. Isolation and characterization of biosurfactants producing.
Hemolysis was used as a criterion for the primary isolation of biosurfactant producing bacteria. The screened bacteria were identified by gcfame analysis as bacillus licheniformis. To confirm the ability of isolates to produce biosurfactant, oil displacement test, measurement of surface tension and e24 test were conducted. Isolation of crude oil degrading marine bacteria and. Isolated microorganisms were identified based on the biochemical characterization as. After screening, candidate biosurfactant producing bacteria were selected for optimization of the physical and. Biosurfactant produced by none of the isolates had hemolytic properties. Isolation and screening of biosurfactant producing. Ten morphologically distinct microbial colonies were isolated and. Isolation of potent biosurfactant producing bacteria from.
Haemolysis has been used as an initial selection criterion for the primary isolation of surfactantproducing bacteria. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. Hassanshahian m 2014 isolation and characterization of biosurfactant producing bacteria from persian gulf bushehr provenance. We successfully isolated three biosurfactant producers. Three isolates designated c1p, nc11p and c1a were selected for this study on the basis of their characteristics and good growth. Isolation and screening of biosurfactants produced by. Isolation and preliminary screening of lignin degrading microbes. Alternative methodology for isolation of biosurfactantproducing bacteria krepsky, n. In article 12 walter, christoph syldatk and rudolf hausmann, 2010. Screening, isolation and characterization of biosurfactant producing. B screening and isolation of biosurfactantproducing bacteria bacteria were isolated from above samples as follows. Sep 15, 2014 in this research six different genus of biosurfactant producing bacteria were isolated from persian gulf at bushehr provenance.
In this research six different genus of biosurfactant producing bacteria were isolated from persian gulf at bushehr provenance. Screening and optimization of biosurfactant production by. In this study, a biosurfactantproducing strain was isolated from a portuguese dairy plant, and the effect of different nitrogen sources on growth and biosurfactant production was evaluated. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Isolation and screening of biosurfactant producing bacteria from oil contaminated soils in iraq doi. The human breast milk samples were collected aseptically and then cultured in mrs agar media.
Pruthi and cameotra 1997 screened biosurfactant producing bacteria by assay the cell surface hydrophobicity. Screening of biosurfactant production was performed using the. Introduction biosurfactants are surface active compound that reduce the. Isolation of biosurfactant producing bacteria from garbage. Fifty five strains had haemolytic activity, among them twelve strains were good biosurfactant producers by measuring surface tension and emulsification activity. The collected soil samples were kept in sterile bags and transported to experimental laboratory within 2 h before sampling. Production and stability studies of the biosurfactant isolated from. The use of selective media containing sucrose 10 g.
The word surfactant is derived from the term surface active agent. Pdf isolation and selection of biosurfactantproducing bacteria. Considering leakage of crude oil from tankers in harbors and other anthropogenic activities, we suspected the occurrence of biosurfactantproducing bacteria at our sampling site ebrahimi et al. The present study demonstrates the isolation and characterization of biosurfactant generating bacteria from human breast milk samples. Screening concepts for the isolation of biosurfactant producing microorganisms. Hydrocarboncontaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains have also been isolated from undisturbed sites. Twenty three morphologically distinct microbial colonies 14 gram positive including one coccus and bacilli. Isolation and characterization of biosurfactant producing bacteria from oil contaminated soil a thesis submitted to the national institute of technology, rourkela in partial fulfillment of the requirements of the degree of bachelor of technology chemical engineering by ajeet kumar soni roll no. May 01, 20 issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Characterization and phylogenetic analysis of biosurfactant. Isolation, screening and characterization of potent biosurfactant producing bacteria from oil contaminated site manish p. Isolation of crude oil degrading marine bacteria and assessment for biosurfactant production 4 of 7 positive for production of biosurfactant and emulsification of toluene table 2.
This was evident as no zone of clearance was observed in the blood agar plates. Tannery effluents provide an environment for growth of different types of microorganisms. May 07, 20 the bacterial colonies were then observed for the presence of clear zonearound the colonies. Abstract biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. Easily share your publications and get them in front of issuus. Distribution of biosurfactantproducing bacteria in undisturbed and. Isolation and selection of new biosurfactant producing.
Nov 23, 2014 the isolation of a new biosurfactant producing bacterium was carried out in soil and water samples collected from jeddah region, ksa. Yeast extract was found to be an essential component for bacterial growth, while peptone is the most important factor for biosurfactant production. Research article isolation, screening and characterization. Isolation and characterization of biosurfactant producing. The present study was focused on isolation of biosurfactant producing bacteria from environmental samples contaminated soil and uncontaminated water and assessing the potential of these isolates.
Isolation of biosurfactant producing bacteria from. Isolation and preliminary screening of lignin degrading. Isolation and characterization of biosurfactant production. Identification of biosurfactant producing bacteria.
Isolation and production of biosurfactant from pseudomonas. Research projects seeking funding isolation and characterization of biosurfactant producing bacteria from oil polluted environment. Isolation and characterization of biosurfactant production under. Characterization and phylogenetic analysis of biosurfactant producing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand. Pdf isolation and selection of biosurfactantproducing. A phylogenetic tree was constructed by combining the results of the present study with a. The methodology was able to isolate biosurfactantproducing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources. Ten selected biosurfactant producing strains were screened for biosurfactant production by numerous tests. A study on biosurfactant production from marine bacteria. In this study biosurfactant producing bacteria were isolated from 50 terrestrial samples collected in areas contaminated with petroleum in several districts in bangkok, thailand. Effects of different ph, salinity and temperature on biosurfactant production were also studied.
Only five strains, all of them haemolytic, tested positive. These clear zones indicate the presence of biosurfactants producing bacteria. These isolates were studied for their biosurfactant producing potentials. Ecofriendly and innovative biocides against biocorrosion. Isolation of biosurfactantproducing marine bacteria and characteristics of selected biosurfactant songklanakarin j. Alternative methodology for isolation of biosurfactant. Enumeration of biosurfactant producing microorganisms from oil contaminated soil in and around bangalore india. Evaluation of screening methods for the isolation of biosurfactant. Antibiotic sensitivity test for biosurfactant producing bacteria 8. Biosurfactant have special advantage over their commercially manufactured counterparts because of its low toxicity, biodegradable nature, and effectiveness at extreme temperature, ph, salinity and ease of synthesis 9.
Isolation, characterization and identification of bacterial. Isolation of biosurfactant producing bacteria from petroleum. Screening and characterization of biosurfactant produced by. Isolation and screening of biosurfactant producing bacteria. Wide biosurfactant application on biorremediation is limited by its high production cost. The objective of the present study is to screen the biosurfactant producing bacteria from soil contaminated with domestic waste water. Pdf isolation of biosurfactant producing bacteria from. Among the various bioactive compounds, biosurfactants bioemulsifiers are attracting major interest and. Nov 11, 2016 hassanshahian m 2014 isolation and characterization of biosurfactant producing bacteria from persian gulf bushehr provenance. After screening, candidate biosurfactantproducing bacteria were selected for optimization of the physical and. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran 2 biotechnology center of iranian research organization for science and technology, iran 3research institute of petroleum industry, tehran, iran.
Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. Isolation of biosurfactant producers, optimization and properties of. We isolated and screened potential biosurfactantproducing bacteria. Therefore, present study focused on the isolation of biosurfactant producing bacteria from. Screening and isolation of biosurfactant producing bacteria from. The isolation of a new biosurfactant producing bacterium was carried out in soil and water samples collected from jeddah region, ksa. Isolation of biosurfactant producing bacteria from garbage soil. Isolation and selection of biosurfactantproducing bacteria. Only 37 of 492 strains of different origins had haemolytic activity.
Surfactants are chemicals that change the properties of water and other fluids. Biosurfuctant, bacteria, bacillus subtilis, 16s rrna. Isolation and screening of biosurfactantproducing bacteria. The sample was taken in sterile polythene bag and was taken to the laboratory and analyzed for the isolation of dye decolorizing bacteria. Isolation and enrichment of biosurfactant producing bacteria two biosurfactant producing bacterial strains were isolated and enriched from diesel oil contaminated soil in dindigul 10. The search for cheaper biossurfactant production alternatives has guided our study. Screening concepts for the isolation of biosurfactant. Lignocellulose is the major structural component of woody plants and nonwoody plants such as grasses and represents a major source of renewable organic matter. Neighborjoining method was used to calculate the evolutionary. Thus the present study was carried out to determine the antimicrobial and emulsifying applications of biosurfactant extracted from halobacterium salinarum isolated from sea water.
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